BD HorizonTM RY610 Rat Anti-Mouse CD3

Description

Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The leukocytes were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon RY610 Rat IgG2b, kappa Isotype Control Cat. No. 571153; Left Plot or BD Horizon RY610 Rat Anti-Mouse CD3 antibody Cat. No. 571224/571289; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The leukocytes were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon RY610 Rat IgG2b, kappa Isotype Control Cat. No. 571153; Left Plot or BD Horizon RY610 Rat Anti-Mouse CD3 antibody Cat. No. 571224/571289; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The leukocytes were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon RY610 Rat IgG2b, kappa Isotype Control Cat. No. 571153; Left Plot or BD Horizon RY610 Rat Anti-Mouse CD3 antibody Cat. No. 571224/571289; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The leukocytes were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon RY610 Rat IgG2b, kappa Isotype Control Cat. No. 571153; Left Plot or BD Horizon RY610 Rat Anti-Mouse CD3 antibody Cat. No. 571224/571289; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The leukocytes were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon RY610 Rat IgG2b, kappa Isotype Control Cat. No. 571153; Left Plot or BD Horizon RY610 Rat Anti-Mouse CD3 antibody Cat. No. 571224/571289; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 Mouse BD Fc Block Cat. No. 553142 and BD Pharmingen Purified Armenian Hamster Anti-Mouse FcgammaRIV CD16-2 Cat. No. 567208 antibodies. The leukocytes were then stained with APC Rat Anti-Mouse CD19 antibody Cat. No. 550992 and with either BD Horizon RY610 Rat IgG2b, kappa Isotype Control Cat. No. 571153; Left Plot or BD Horizon RY610 Rat Anti-Mouse CD3 antibody Cat. No. 571224/571289; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD3 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.