BD HorizonTM RY610 Rat Anti-Mouse Ly-6C

Description

Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY610 Rat IgM, kappa Isotype Control Cat. No. 571150; Left Plot or BD Horizon RY610 Rat Anti-Mouse Ly-6C antibody Cat. No. 571148/571149; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Flow cytometry and data analysis were performed using BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY610 Rat IgM, kappa Isotype Control Cat. No. 571150; Left Plot or BD Horizon RY610 Rat Anti-Mouse Ly-6C antibody Cat. No. 571148/571149; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Flow cytometry and data analysis were performed using BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY610 Rat IgM, kappa Isotype Control Cat. No. 571150; Left Plot or BD Horizon RY610 Rat Anti-Mouse Ly-6C antibody Cat. No. 571148/571149; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Flow cytometry and data analysis were performed using BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY610 Rat IgM, kappa Isotype Control Cat. No. 571150; Left Plot or BD Horizon RY610 Rat Anti-Mouse Ly-6C antibody Cat. No. 571148/571149; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Flow cytometry and data analysis were performed using BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY610 Rat IgM, kappa Isotype Control Cat. No. 571150; Left Plot or BD Horizon RY610 Rat Anti-Mouse Ly-6C antibody Cat. No. 571148/571149; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Flow cytometry and data analysis were performed using BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY610 Rat IgM, kappa Isotype Control Cat. No. 571150; Left Plot or BD Horizon RY610 Rat Anti-Mouse Ly-6C antibody Cat. No. 571148/571149; Right Plot at 1 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Flow cytometry and data analysis were performed using BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.