BD HorizonTM RY655 Mouse Anti-Human CD45RO

Description

Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885; Bottom Plots and with either BD Horizon RY655 Mouse IgG2a, kappa Isotype Control Cat. No. 572361; Left Plots or BD Horizon RY655 Mouse Anti-Human CD45RO antibody Cat. No. 572355/572356; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and compensated using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing the coexpression of CD45RO or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885; Bottom Plots and with either BD Horizon RY655 Mouse IgG2a, kappa Isotype Control Cat. No. 572361; Left Plots or BD Horizon RY655 Mouse Anti-Human CD45RO antibody Cat. No. 572355/572356; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and compensated using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing the coexpression of CD45RO or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD45RA antibody Cat. No. 562885; Bottom Plots and with either BD Horizon RY655 Mouse IgG2a, kappa Isotype Control Cat. No. 572361; Left Plots or BD Horizon RY655 Mouse Anti-Human CD45RO antibody Cat. No. 572355/572356; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer and compensated using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the side and forward light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing the coexpression of CD45RO or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.