BD HorizonTM RY655 Mouse Anti-Human Granzyme B

Description

Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human Granzyme B antibody Cat. No. 572353/572354; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human Granzyme B antibody Cat. No. 572353/572354; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human Granzyme B antibody Cat. No. 572353/572354; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human Granzyme B antibody Cat. No. 572353/572354; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human Granzyme B antibody Cat. No. 572353/572354; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes . Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse Anti-Human CD8 antibody Cat. No. 562428 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human Granzyme B antibody Cat. No. 572353/572354; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.