BD HorizonTM RY655 Mouse Anti-Human IFN-gamma

Description

Two-color flow cytometric analysis of IFN-gamma expressed in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were washed and then permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human IFN-gamma antibody Cat. No. 572333/572334; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of IFN-gamma expressed in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were washed and then permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human IFN-gamma antibody Cat. No. 572333/572334; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of IFN-gamma expressed in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were washed and then permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human IFN-gamma antibody Cat. No. 572333/572334; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Two-color flow cytometric analysis of IFN-gamma expressed in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were washed and then permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human IFN-gamma antibody Cat. No. 572333/572334; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Two-color flow cytometric analysis of IFN-gamma expressed in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were washed and then permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human IFN-gamma antibody Cat. No. 572333/572334; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of IFN-gamma expressed in stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 6 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were washed and then permeabilized with and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY655 Mouse IgG1, kappa Isotype Control Cat. No. 572346; Left Plot or BD Horizon RY655 Mouse Anti-Human IFN-gamma antibody Cat. No. 572333/572334; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IFN-gamma or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software.