BD HorizonTM RY655 Rat Anti-Mouse CD4

Description

Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY655 Rat IgG2b, kappa Isotype Control Cat. No. 572348; Left Plot or BD Horizon RY655 Rat Anti-Mouse CD4 antibody Cat. No. 572342/572343; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY655 Rat IgG2b, kappa Isotype Control Cat. No. 572348; Left Plot or BD Horizon RY655 Rat Anti-Mouse CD4 antibody Cat. No. 572342/572343; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY655 Rat IgG2b, kappa Isotype Control Cat. No. 572348; Left Plot or BD Horizon RY655 Rat Anti-Mouse CD4 antibody Cat. No. 572342/572343; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY655 Rat IgG2b, kappa Isotype Control Cat. No. 572348; Left Plot or BD Horizon RY655 Rat Anti-Mouse CD4 antibody Cat. No. 572342/572343; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY655 Rat IgG2b, kappa Isotype Control Cat. No. 572348; Left Plot or BD Horizon RY655 Rat Anti-Mouse CD4 antibody Cat. No. 572342/572343; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD4 expression on Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BV421 Hamster Anti-Mouse CD3e antibody Cat. No. 562600 and with either BD Horizon RY655 Rat IgG2b, kappa Isotype Control Cat. No. 572348; Left Plot or BD Horizon RY655 Rat Anti-Mouse CD4 antibody Cat. No. 572342/572343; Right Plot at 0.25 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer System and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.