BD HorizonTM RY655 Streptavidin

Description

Multiparameter flow cytometric analyses using BD Horizon RY655 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and analyzed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RY655 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and analyzed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RY655 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and analyzed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analyses using BD Horizon RY655 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and analyzed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analyses using BD Horizon RY655 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and analyzed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RY655 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The cells were then washed and secondarily stained with BD Horizon RY655 Streptavidin Cat. No. 572362 at 0.125 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and analyzed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.