BD HorizonTM RY703 Hamster Anti-Mouse CD3e

Description

Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic T lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either BD Horizon RY703 Hamster IgG1, kappa Isotype control Cat. No. 571631; Left Plot or BD Horizon RY703 Hamster Anti-Mouse CD3e antibody Cat. No. 571788/571839; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic T lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either BD Horizon RY703 Hamster IgG1, kappa Isotype control Cat. No. 571631; Left Plot or BD Horizon RY703 Hamster Anti-Mouse CD3e antibody Cat. No. 571788/571839; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic T lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either BD Horizon RY703 Hamster IgG1, kappa Isotype control Cat. No. 571631; Left Plot or BD Horizon RY703 Hamster Anti-Mouse CD3e antibody Cat. No. 571788/571839; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic T lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either BD Horizon RY703 Hamster IgG1, kappa Isotype control Cat. No. 571631; Left Plot or BD Horizon RY703 Hamster Anti-Mouse CD3e antibody Cat. No. 571788/571839; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic T lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either BD Horizon RY703 Hamster IgG1, kappa Isotype control Cat. No. 571631; Left Plot or BD Horizon RY703 Hamster Anti-Mouse CD3e antibody Cat. No. 571788/571839; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD3e expression on viable Mouse splenic T lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with FITC Rat Anti-Mouse CD4 Cat. No. 553047 and FITC Rat Anti-Mouse CD8a Cat. No. 553030 antibodies and with either BD Horizon RY703 Hamster IgG1, kappa Isotype control Cat. No. 571631; Left Plot or BD Horizon RY703 Hamster Anti-Mouse CD3e antibody Cat. No. 571788/571839; Right Plot at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of CD3e or Ig Isotype Control staining versus CD4 and CD8a was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.