BD HorizonTM RY703 Mouse Anti-Human CD127 (IL-7 Receptor alpha)

Description

Multicolor flow cytometric analysis of CD127 IL-7 Receptor alpha expression on Human peripheral blood leukocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 , and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti- Human CD127 IL-7 Receptor alpha antibody Cat. No. 571814/571865; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD127 IL-7 Receptor alpha or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Multicolor flow cytometric analysis of CD127 IL-7 Receptor alpha expression on Human peripheral blood leukocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 , and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti- Human CD127 IL-7 Receptor alpha antibody Cat. No. 571814/571865; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD127 IL-7 Receptor alpha or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Multicolor flow cytometric analysis of CD127 IL-7 Receptor alpha expression on Human peripheral blood leukocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 , and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti- Human CD127 IL-7 Receptor alpha antibody Cat. No. 571814/571865; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD127 IL-7 Receptor alpha or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD127 IL-7 Receptor alpha expression on Human peripheral blood leukocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 , and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti- Human CD127 IL-7 Receptor alpha antibody Cat. No. 571814/571865; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD127 IL-7 Receptor alpha or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Show More Multicolor flow cytometric analysis of CD127 IL-7 Receptor alpha expression on Human peripheral blood leukocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 , and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti- Human CD127 IL-7 Receptor alpha antibody Cat. No. 571814/571865; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD127 IL-7 Receptor alpha or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Multicolor flow cytometric analysis of CD127 IL-7 Receptor alpha expression on Human peripheral blood leukocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 , and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti- Human CD127 IL-7 Receptor alpha antibody Cat. No. 571814/571865; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD127 IL-7 Receptor alpha or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software.