BD HorizonTM RY703 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Two-color flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571443/571444; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin. The cells were then stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Two-color flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571443/571444; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Show More Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin. The cells were then stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Show More Two-color flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD4 antibody Cat. No. 555349/561840 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571443/571444; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin. The cells were then stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software.