BD HorizonTM RY703 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 and stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571822/571873; Middle Plot . Right Plots - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 and stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571822/571873; Middle Plot . Right Plots - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 and stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571822/571873; Middle Plot . Right Plots - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Show More Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 and stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571822/571873; Middle Plot . Right Plots - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Show More Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 and stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571822/571873; Middle Plot . Right Plots - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts. Flow cytometric analysis of CD25 IL-2 Receptor alpha expression on unstimulated and PHA-stimulated Human peripheral blood lymphocytes. Left and Middle Plots - Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564220 and stained with BD Horizon BUV395 Mouse Anti-Human CD4 antibody Cat. No. 564724 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 571822/571873; Middle Plot . Right Plots - Human peripheral blood mononuclear cells PBMC were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphoblasts.