BD HorizonTM RY703 Mouse Anti-Human CD279 (PD-1)

Description

Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/563548 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 571439/571440; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/563548 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 571439/571440; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/563548 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 571439/571440; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/563548 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 571439/571440; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/563548 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 571439/571440; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of CD279 PD-1 expression on Human peripheral blood lymphocytes. Human whole blood was stained with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546/563548 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD279 PD-1 antibody Cat. No. 571439/571440; Right Plot . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD279 PD-1 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software.