BD HorizonTM RY703 Mouse Anti-Human CD4

Description

Flow cytometric analysis of CD4 expression on Rhesus macaque peripheral blood lymphocytes. Rhesus macaque whole blood was stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD4 antibody Cat. No. 571820/571871; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histogram showing CD4 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD4 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD4 antibody Cat. No. 571820/571871; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Flow cytometric analysis of CD4 expression on Rhesus macaque peripheral blood lymphocytes. Rhesus macaque whole blood was stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD4 antibody Cat. No. 571820/571871; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histogram showing CD4 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD4 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD4 antibody Cat. No. 571820/571871; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Show More Flow cytometric analysis of CD4 expression on Rhesus macaque peripheral blood lymphocytes. Rhesus macaque whole blood was stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; dashed line histogram or BD Horizon RY703 Mouse Anti-Human CD4 antibody Cat. No. 571820/571871; solid line histogram . The erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The fluorescence histogram showing CD4 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD4 expression on Human peripheral blood leukocyte populations. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 and stained with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human CD4 antibody Cat. No. 571820/571871; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocyte populations. Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software.