BD HorizonTM RY703 Mouse Anti-Human CD45RA

Description

Multiparameter flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with FITC Mouse Anti-Human CD45RO antibody Cat. No. 555492 and with either BD Horizon RY703 Mouse IgG2b, kappa Isotype Control Cat. No. 571460; Left Plots or BD Horizon RY703 Mouse Anti-Human CD45RA antibody Cat. No. 571811/571862; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of either CD45RA or Ig Isotype control staining versus CD45RO Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with FITC Mouse Anti-Human CD45RO antibody Cat. No. 555492 and with either BD Horizon RY703 Mouse IgG2b, kappa Isotype Control Cat. No. 571460; Left Plots or BD Horizon RY703 Mouse Anti-Human CD45RA antibody Cat. No. 571811/571862; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of either CD45RA or Ig Isotype control staining versus CD45RO Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD45RA expression on Human peripheral blood leukocytes. Human whole blood was stained with FITC Mouse Anti-Human CD45RO antibody Cat. No. 555492 and with either BD Horizon RY703 Mouse IgG2b, kappa Isotype Control Cat. No. 571460; Left Plots or BD Horizon RY703 Mouse Anti-Human CD45RA antibody Cat. No. 571811/571862; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . Bivariate pseudocolor density plots showing the correlated expression of either CD45RA or Ig Isotype control staining versus CD45RO Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leukocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.