BD HorizonTM RY703 Mouse Anti-Human CD56 (NCAM-1)

Description

Multiparameter flow cytometric analysis of CD56 NCAM-1 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with FITC Mouse Anti-Human CD16 antibody Cat. No. 555406; Bottom Plots and with either BD Horizon RY703 Mouse IgG2b, kappa Isotype Control Cat. No. 571460; Left Plots or BD Horizon RY703 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 571828/571879; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of CD56 NCAM-1 or Ig Isotype control staining versus side light-scatter SSC-A signals or CD16 were derived from gated events with the forward and side light-scatter characteristics of viable leukocytes Top Plots or lymphocytes Bottom Plots . Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Multiparameter flow cytometric analysis of CD56 NCAM-1 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with FITC Mouse Anti-Human CD16 antibody Cat. No. 555406; Bottom Plots and with either BD Horizon RY703 Mouse IgG2b, kappa Isotype Control Cat. No. 571460; Left Plots or BD Horizon RY703 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 571828/571879; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of CD56 NCAM-1 or Ig Isotype control staining versus side light-scatter SSC-A signals or CD16 were derived from gated events with the forward and side light-scatter characteristics of viable leukocytes Top Plots or lymphocytes Bottom Plots . Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD56 NCAM-1 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 , and stained with FITC Mouse Anti-Human CD16 antibody Cat. No. 555406; Bottom Plots and with either BD Horizon RY703 Mouse IgG2b, kappa Isotype Control Cat. No. 571460; Left Plots or BD Horizon RY703 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 571828/571879; Right Plots . The bivariate pseudocolor density plots showing the correlated expression of CD56 NCAM-1 or Ig Isotype control staining versus side light-scatter SSC-A signals or CD16 were derived from gated events with the forward and side light-scatter characteristics of viable leukocytes Top Plots or lymphocytes Bottom Plots . Flow cytometry and data analysis were performed using a 5-laser Flow Cytometer System and FlowJo Software.