BD HorizonTM RY703 Mouse Anti-Human Granzyme B

Description

Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human Granzyme B antibody Cat. No. 571461/571462; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human Granzyme B antibody Cat. No. 571461/571462; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human Granzyme B antibody Cat. No. 571461/571462; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human Granzyme B antibody Cat. No. 571461/571462; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human Granzyme B antibody Cat. No. 571461/571462; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Two-color flow cytometric analysis of Granzyme B expression in Human peripheral blood lymphocytes. Human whole blood was fixed with BD Lyse/Fix Buffer Cat. No. 558049 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with APC Mouse Anti-Human CD8 antibody Cat. No. 561953/555369 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-Human Granzyme B antibody Cat. No. 571461/571462; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of Granzyme B or Ig Isotype control staining versus CD8 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software.