BD HorizonTM RY703 Mouse Anti-T-bet

Description

Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-T-bet antibody Cat. No. 571477/571478; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-T-bet antibody Cat. No. 571477/571478; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-T-bet antibody Cat. No. 571477/571478; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-T-bet antibody Cat. No. 571477/571478; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-T-bet antibody Cat. No. 571477/571478; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Two-color flow cytometric analysis of T-bet expression in Human peripheral blood lymphocytes. Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leukocytes. The leukocytes were permeabilized by treatment with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with BD Pharmingen APC Mouse Anti-Human CD3 antibody Cat. No. 561811 and with either BD Horizon RY703 Mouse IgG1, kappa Isotype Control Cat. No. 571453; Left Plot or BD Horizon RY703 Mouse Anti-T-bet antibody Cat. No. 571477/571478; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of T-bet or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software.