BD HorizonTM RY703 Rat Anti-Mouse CD4

Description

Multicolor flow cytometric analysis of CD4 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD4 antibody Cat. No. 571611/571617; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD4 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD4 antibody Cat. No. 571611/571617; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD4 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD4 antibody Cat. No. 571611/571617; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD4 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD4 antibody Cat. No. 571611/571617; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD4 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD4 antibody Cat. No. 571611/571617; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD4 expression on viable Mouse splenic leukocytes. BALB/c Mouse splenocytes were treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes, washed, and preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD4 antibody Cat. No. 571611/571617; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.