BD HorizonTM RY703 Rat Anti-Mouse CD8a

Description

Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD8a antibody Cat. No. 571447/571448; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD8a antibody Cat. No. 571447/571448; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD8a antibody Cat. No. 571447/571448; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD8a antibody Cat. No. 571447/571448; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD8a antibody Cat. No. 571447/571448; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD8a expression on Mouse splenic leukocytes. BALB/c mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/32 antibody Mouse Fc Block Cat. No. 553142 . The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and with either BD Horizon RY703 Rat IgG2a, kappa Isotype Control Cat. No. 571454; Left Plot or BD Horizon RY703 Rat Anti-Mouse CD8a antibody Cat. No. 571447/571448; Right Plot at 0.5 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD8a or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.