BD HorizonTM RY743 Mouse Anti-Human CD25 (IL-2 Receptor alpha)

Description

Multiparameter flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes . Human whole blood was stained with BD Horizon RB744 Mouse Anti-Human CD4 antibody Cat. No. 570466 and with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat. No. 572209; Left Plot or BD Horizon RY743 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 572198/572199; Right Plot . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat No. 572209; dashed line histogram or BD Horizon RY743 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 572198/572199; solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Multiparameter flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes . Human whole blood was stained with BD Horizon RB744 Mouse Anti-Human CD4 antibody Cat. No. 570466 and with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat. No. 572209; Left Plot or BD Horizon RY743 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 572198/572199; Right Plot . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Show More Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat No. 572209; dashed line histogram or BD Horizon RY743 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 572198/572199; solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Show More Multiparameter flow cytometric analysis for CD25 IL-2 Receptor alpha on unstimulated Human peripheral blood lymphocytes . Human whole blood was stained with BD Horizon RB744 Mouse Anti-Human CD4 antibody Cat. No. 570466 and with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat. No. 572209; Left Plot or BD Horizon RY743 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat. No. 572198/572199; Right Plot . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plot showing the correlated expression of CD25 IL-2 Receptor alpha or Ig Isotype control staining versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software. Flow cytometric analysis for CD25 IL-2 Receptor alpha on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stimulated for 3 days with Phytohemagglutinin PHA . The cells were stained with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat No. 572209; dashed line histogram or BD Horizon RY743 Mouse Anti-Human CD25 IL-2 Receptor alpha antibody Cat No. 572198/572199; solid line histogram . The fluorescence histogram showing CD25 IL-2 Receptor alpha expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Flow Cytometer System and FlowJo Software.