
572226 · 25 Tests
BDB Bioscience · Cat: 572226
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Description
Flow cytometric analysis of Human CD274 PD-L1 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days. The cells were then stained with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat. No. 572209; dashed line histogram or BD Horizon RY743 Mouse Anti-Human CD274 PD-L1 antibody Cat. No. 572225/572226, solid line histogram . The fluorescence histogram showing the expression of CD274 PD-L1 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Flow cytometric analysis of Human CD274 PD-L1 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days. The cells were then stained with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat. No. 572209; dashed line histogram or BD Horizon RY743 Mouse Anti-Human CD274 PD-L1 antibody Cat. No. 572225/572226, solid line histogram . The fluorescence histogram showing the expression of CD274 PD-L1 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Show More Flow cytometric analysis of Human CD274 PD-L1 expression on activated Human lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated with Phytohemagglutinin PHA for 3 days. The cells were then stained with either BD Horizon RY743 Mouse IgG1, kappa Isotype Control Cat. No. 572209; dashed line histogram or BD Horizon RY743 Mouse Anti-Human CD274 PD-L1 antibody Cat. No. 572225/572226, solid line histogram . The fluorescence histogram showing the expression of CD274 PD-L1 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of activated viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software.