BD HorizonTM RY743 Rat Anti-Human IL-2

Description

Two-color flow cytometric analysis of IL-2 in stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY743 Rat IgG2a, kappa Isotype Control Cat. No. 572210; Left Plot or BD Horizon RY743 Rat Anti-Human IL-2 antibody Cat. No. 572235/572236; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software Two-color flow cytometric analysis of IL-2 in stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY743 Rat IgG2a, kappa Isotype Control Cat. No. 572210; Left Plot or BD Horizon RY743 Rat Anti-Human IL-2 antibody Cat. No. 572235/572236; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software Two-color flow cytometric analysis of IL-2 in stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY743 Rat IgG2a, kappa Isotype Control Cat. No. 572210; Left Plot or BD Horizon RY743 Rat Anti-Human IL-2 antibody Cat. No. 572235/572236; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software Show More Two-color flow cytometric analysis of IL-2 in stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY743 Rat IgG2a, kappa Isotype Control Cat. No. 572210; Left Plot or BD Horizon RY743 Rat Anti-Human IL-2 antibody Cat. No. 572235/572236; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software Show More Two-color flow cytometric analysis of IL-2 in stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY743 Rat IgG2a, kappa Isotype Control Cat. No. 572210; Left Plot or BD Horizon RY743 Rat Anti-Human IL-2 antibody Cat. No. 572235/572236; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software Two-color flow cytometric analysis of IL-2 in stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , and fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BUV395 Mouse Anti-Human CD3 antibody Cat. No. 563546 and with either BD Horizon RY743 Rat IgG2a, kappa Isotype Control Cat. No. 572210; Left Plot or BD Horizon RY743 Rat Anti-Human IL-2 antibody Cat. No. 572235/572236; Right Plot . The bivariate pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Samples were acquired using a BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software