BD HorizonTM RY743 Streptavidin

Description

Multiparameter flow cytometric analyses using BD Horizon RY743 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RY743 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RY743 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analyses using BD Horizon RY743 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analyses using BD Horizon RY743 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analyses using BD Horizon RY743 Streptavidin Left Plot: CD45R/B220 expression on viable Mouse splenic leukocytes. Mouse splenocytes were either labeled with Biotin Rat IgG2a, kappa Isotype Control Cat. No. 553928; dashed line histogram or with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553086; solid line histogram . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Right Plot: CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. The leukocytes were either labeled with Biotin Mouse IgG1, kappa Isotype Control Cat. No. 569597; dashed line histogram or with Biotin Mouse Anti-Human CD3 antibody solid line histogram; Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY743 Streptavidin Cat. No. 572220 at 0.25 mug/test. The fluorescence histogram showing CD3 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.