
571396 · 100 Tests
BDB Bioscience · Cat: 571396
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Flow cytometric analysis of CD69 expression on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 24 hours with Phytohemagglutinin PHA . The cells were then stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD69 antibody Cat. No. 571396/571397; solid line histogram . The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable activated lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software. Flow cytometric analysis of CD69 expression on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 24 hours with Phytohemagglutinin PHA . The cells were then stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD69 antibody Cat. No. 571396/571397; solid line histogram . The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable activated lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software. Show More Flow cytometric analysis of CD69 expression on stimulated Human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells PBMC were stimulated for 24 hours with Phytohemagglutinin PHA . The cells were then stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD69 antibody Cat. No. 571396/571397; solid line histogram . The fluorescence histogram showing CD69 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable activated lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software.