BD HorizonTM RY775 Mouse Anti-Human CD86 (B7-2)

Description

Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571609/571615; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing CD86 B7-2 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571609/571615; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571609/571615; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing CD86 B7-2 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571609/571615; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of CD86 B7-2 expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571609/571615; Right Plot . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plot showing CD86 B7-2 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD86 B7-2 expression on Daudi cells. Cells from the Human Daudi Burkitts lymphoma, ATCC CRL-213 cell line were stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; dashed line histogram or BD Horizon RY775 Mouse Anti-Human CD86 B7-2 antibody Cat. No. 571609/571615; solid line histogram . DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.