BD HorizonTM RY775 Mouse Anti-Human IgG

Description

Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium overnight, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 to minimize subsequent nonspecific immunofluorescent staining. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human IgG antibody Cat. No. 571641/571720; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium overnight, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 to minimize subsequent nonspecific immunofluorescent staining. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human IgG antibody Cat. No. 571641/571720; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium overnight, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 to minimize subsequent nonspecific immunofluorescent staining. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human IgG antibody Cat. No. 571641/571720; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium overnight, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 to minimize subsequent nonspecific immunofluorescent staining. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human IgG antibody Cat. No. 571641/571720; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium overnight, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 to minimize subsequent nonspecific immunofluorescent staining. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human IgG antibody Cat. No. 571641/571720; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were cultured in complete tissue culture medium overnight, washed, and then preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219 to minimize subsequent nonspecific immunofluorescent staining. The cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440 and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plot or BD Horizon RY775 Mouse Anti-Human IgG antibody Cat. No. 571641/571720; Right Plot . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.