BD HorizonTM RY775 Mouse Anti-Human TIM-3 (CD366)

Description

Multiparameter flow cytometric analysis of TIM-3 CD366 expression on human peripheral blood leukocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 562751; Lower Plots and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plots or BD Horizon RY775 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 571402/571403; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Top Plots - The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leukocyte populations. Lower Plots - The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Multiparameter flow cytometric analysis of TIM-3 CD366 expression on human peripheral blood leukocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 562751; Lower Plots and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plots or BD Horizon RY775 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 571402/571403; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Top Plots - The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leukocyte populations. Lower Plots - The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Show More Multiparameter flow cytometric analysis of TIM-3 CD366 expression on human peripheral blood leukocytes. Human whole blood was stained with BD Horizon BV421 Mouse Anti-Human CD56 NCAM-1 antibody Cat. No. 562751; Lower Plots and with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plots or BD Horizon RY775 Mouse Anti-Human TIM-3 CD366 antibody Cat. No. 571402/571403; Right Plots . The erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Top Plots - The bivariate pseudocolor density plot showing TIM-3 CD366 expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side-light scatter characteristics of viable leukocyte populations. Lower Plots - The bivariate pseudocolor density plot showing the correlated expression of TIM-3 CD366 or Ig Isotype control staining versus CD56 NCAM-1 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software.