BD HorizonTM RY775 Mouse Anti-Ki-67

Description

Multiparameter flow cytometric analysis of Ki-67 expression in noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells containing lymphocytes Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plots or BD Horizon RY775 Mouse Anti-Ki-67 antibody Cat. No. 571398/571399; Right Plots and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DNA DAPI versus Ki-67 levels or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC Top Plots or MOLT-4 cells Bottom Plots .Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Multiparameter flow cytometric analysis of Ki-67 expression in noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells containing lymphocytes Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plots or BD Horizon RY775 Mouse Anti-Ki-67 antibody Cat. No. 571398/571399; Right Plots and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DNA DAPI versus Ki-67 levels or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC Top Plots or MOLT-4 cells Bottom Plots .Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Show More Multiparameter flow cytometric analysis of Ki-67 expression in noncycling Human peripheral blood lymphocytes or proliferating Human MOLT-4 cells. Noncycling Human peripheral blood mononuclear cells containing lymphocytes Top Plots or proliferating cells from the Human MOLT-4 T lymphoblastic leukemia, ATCC CRL-1582 cell line Bottom Plots were permeabilized and fixed with 70 % ice-cold ethanol. The cells were washed twice with BD Pharmingen Stain Buffer FBS Cat. No. 554656 , stained with either BD Horizon RY775 Mouse IgG1, kappa Isotype Control Cat. No. 571408; Left Plots or BD Horizon RY775 Mouse Anti-Ki-67 antibody Cat. No. 571398/571399; Right Plots and counterstained with BD Pharmingen DAPI Solution Cat. No. 564907 to stain DNA. Bivariate pseudocolor density plots showing the correlated expression of DNA DAPI versus Ki-67 levels or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact PBMC Top Plots or MOLT-4 cells Bottom Plots .Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software.