BD HorizonTM RY775 Rat Anti-Mouse CD45R (B220)

Description

Multicolor flow cytometric analysis of CD45R B220 expression on Mouse splenic leukocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Cat. No. 553142 .The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and either BD Horizon RY775 Rat IgG2a, kappa Isotype Control Cat. No. 571409; Left Plot or BD Horizon RY775 Rat Anti-Mouse CD45R B220 antibody Cat. No. 571680/571753; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells before analysis. The bivariate pseudocolor density plot showing the expression of CD45R B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometric analysis was performed using a 5-laser flow cytometer system and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R B220 expression on Mouse splenic leukocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Cat. No. 553142 .The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and either BD Horizon RY775 Rat IgG2a, kappa Isotype Control Cat. No. 571409; Left Plot or BD Horizon RY775 Rat Anti-Mouse CD45R B220 antibody Cat. No. 571680/571753; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells before analysis. The bivariate pseudocolor density plot showing the expression of CD45R B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometric analysis was performed using a 5-laser flow cytometer system and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R B220 expression on Mouse splenic leukocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Cat. No. 553142 .The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and either BD Horizon RY775 Rat IgG2a, kappa Isotype Control Cat. No. 571409; Left Plot or BD Horizon RY775 Rat Anti-Mouse CD45R B220 antibody Cat. No. 571680/571753; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells before analysis. The bivariate pseudocolor density plot showing the expression of CD45R B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometric analysis was performed using a 5-laser flow cytometer system and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD45R B220 expression on Mouse splenic leukocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Cat. No. 553142 .The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and either BD Horizon RY775 Rat IgG2a, kappa Isotype Control Cat. No. 571409; Left Plot or BD Horizon RY775 Rat Anti-Mouse CD45R B220 antibody Cat. No. 571680/571753; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells before analysis. The bivariate pseudocolor density plot showing the expression of CD45R B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometric analysis was performed using a 5-laser flow cytometer system and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD45R B220 expression on Mouse splenic leukocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Cat. No. 553142 .The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and either BD Horizon RY775 Rat IgG2a, kappa Isotype Control Cat. No. 571409; Left Plot or BD Horizon RY775 Rat Anti-Mouse CD45R B220 antibody Cat. No. 571680/571753; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells before analysis. The bivariate pseudocolor density plot showing the expression of CD45R B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometric analysis was performed using a 5-laser flow cytometer system and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD45R B220 expression on Mouse splenic leukocytes. Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Cat. No. 553142 .The cells were then stained with BD Horizon BUV395 Hamster Anti-Mouse CD3e antibody Cat. No. 563565 and either BD Horizon RY775 Rat IgG2a, kappa Isotype Control Cat. No. 571409; Left Plot or BD Horizon RY775 Rat Anti-Mouse CD45R B220 antibody Cat. No. 571680/571753; Right Plot at 0.125 mug/test. DAPI Solution Cat. No. 564907 was added to cells before analysis. The bivariate pseudocolor density plot showing the expression of CD45R B220 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometric analysis was performed using a 5-laser flow cytometer system and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.