BD HorizonTM RY775 Rat Anti-Mouse Ly-6C

Description

Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY775 Rat IgM, kappa Isotype Control Cat. No. 571413; Left Plot or BD Horizon RY775 Rat Anti-Mouse Ly-6C antibody Cat. No. 571411/571412; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY775 Rat IgM, kappa Isotype Control Cat. No. 571413; Left Plot or BD Horizon RY775 Rat Anti-Mouse Ly-6C antibody Cat. No. 571411/571412; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY775 Rat IgM, kappa Isotype Control Cat. No. 571413; Left Plot or BD Horizon RY775 Rat Anti-Mouse Ly-6C antibody Cat. No. 571411/571412; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY775 Rat IgM, kappa Isotype Control Cat. No. 571413; Left Plot or BD Horizon RY775 Rat Anti-Mouse Ly-6C antibody Cat. No. 571411/571412; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY775 Rat IgM, kappa Isotype Control Cat. No. 571413; Left Plot or BD Horizon RY775 Rat Anti-Mouse Ly-6C antibody Cat. No. 571411/571412; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of Ly-6C expression on Mouse splenic leucocytes. BALB/c Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with BD Horizon BUV395 Rat Anti-Mouse CD8a antibody Cat. No. 563786 and with either BD Horizon RY775 Rat IgM, kappa Isotype Control Cat. No. 571413; Left Plot or BD Horizon RY775 Rat Anti-Mouse Ly-6C antibody Cat. No. 571411/571412; Right Plot at 1.0 mug/test. The bivariate pseudocolor density plot showing the correlated expression of Ly-6C or Ig Isotype control staining versus CD8a was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer System and were spectrally unmixed using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.