BD HorizonTM RY775 Streptavidin

Description

Flow cytometric analysis of CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. Leukocytes were then either not labeled dashed line histogram or labeled solid line histogram with Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY775 Streptavidin Cat. No. 571421 at 0.25 mug/test. The fluorescence histogram showing the expression of CD3 or unlabeled control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45R/B220 expression on Mouse splenic leukocytes. Mouse splenic leukocytes were either not labeled dashed line histogram or labeled solid line histogram with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086 . The cells were then washed and secondarily stained with BD Horizon RY775 Streptavidin Cat. No. 571421 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or unlabeled control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. Leukocytes were then either not labeled dashed line histogram or labeled solid line histogram with Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY775 Streptavidin Cat. No. 571421 at 0.25 mug/test. The fluorescence histogram showing the expression of CD3 or unlabeled control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD45R/B220 expression on Mouse splenic leukocytes. Mouse splenic leukocytes were either not labeled dashed line histogram or labeled solid line histogram with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086 . The cells were then washed and secondarily stained with BD Horizon RY775 Streptavidin Cat. No. 571421 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or unlabeled control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD3 expression on Human peripheral blood lymphocytes. Human whole blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to lyse erythrocytes. Leukocytes were then either not labeled dashed line histogram or labeled solid line histogram with Biotin Mouse Anti-Human CD3 antibody Cat. No. 555331 . The cells were then washed and secondarily stained with BD Horizon RY775 Streptavidin Cat. No. 571421 at 0.25 mug/test. The fluorescence histogram showing the expression of CD3 or unlabeled control staining was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD45R/B220 expression on Mouse splenic leukocytes. Mouse splenic leukocytes were either not labeled dashed line histogram or labeled solid line histogram with Biotin Rat Anti-Mouse CD45R/B220 antibody Cat. No. 553085/553086 . The cells were then washed and secondarily stained with BD Horizon RY775 Streptavidin Cat. No. 571421 at 0.25 mug/test. The fluorescence histogram showing CD45R/B220 expression or unlabeled control staining was derived from gated events with the forward and side light-scatter characteristics of viable splenic leukocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed with autofluorescence using FlowJo v10.10 Software. Data shown on this Technical Data Sheet are not lot specific.