BD HorizonTM V450 Mouse anti-BrdU

Description

Flow cytometric analysis of DNA synthesis by TK-1 cells using BD Horizon V450 anti-BrdU antibody Cat. No. 560810 . TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the BD Horizon V450 anti-BrdU antibody followed by the DNA-specific dye, 7-AAD 7-AAD Staining Solution, Cat. No. 559925 . Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSR II System. Flow cytometric analysis of DNA synthesis by TK-1 cells using BD Horizon V450 anti-BrdU antibody Cat. No. 560810 . TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the BD Horizon V450 anti-BrdU antibody followed by the DNA-specific dye, 7-AAD 7-AAD Staining Solution, Cat. No. 559925 . Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSR II System. Flow cytometric analysis of DNA synthesis by TK-1 cells using BD Horizon V450 anti-BrdU antibody Cat. No. 560810 . TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the BD Horizon V450 anti-BrdU antibody followed by the DNA-specific dye, 7-AAD 7-AAD Staining Solution, Cat. No. 559925 . Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSR II System. Show More Flow cytometric analysis of DNA synthesis by TK-1 cells using BD Horizon V450 anti-BrdU antibody Cat. No. 560810 . TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the BD Horizon V450 anti-BrdU antibody followed by the DNA-specific dye, 7-AAD 7-AAD Staining Solution, Cat. No. 559925 . Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSR II System. Show More Flow cytometric analysis of DNA synthesis by TK-1 cells using BD Horizon V450 anti-BrdU antibody Cat. No. 560810 . TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the BD Horizon V450 anti-BrdU antibody followed by the DNA-specific dye, 7-AAD 7-AAD Staining Solution, Cat. No. 559925 . Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSR II System. Flow cytometric analysis of DNA synthesis by TK-1 cells using BD Horizon V450 anti-BrdU antibody Cat. No. 560810 . TK-1 cells were either pulsed with 50 muM BrdU for 1 hour left panel or were not pulsed right panel . Staining was performed using the procedure from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . The permeabilized cells were stained with the BD Horizon V450 anti-BrdU antibody followed by the DNA-specific dye, 7-AAD 7-AAD Staining Solution, Cat. No. 559925 . Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD vs BrdU were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed with doublet discrimination using a BD LSR II System.