BD HorizonTM V450 Mouse anti-Human Granzyme B

Description

Flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 and either BD Horizon V450 Mouse Anti-Human Granzyme B antibody Cat. No.561151; Left Panel or BD Horizon V450 Mouse IgG1 Isotype Control Cat. No.560373; Right Panel Two-color flow cytometric dot plots showing the correlated expression patterns of CD8 and Granzyme B or Mouse IgG1 Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 and either BD Horizon V450 Mouse Anti-Human Granzyme B antibody Cat. No.561151; Left Panel or BD Horizon V450 Mouse IgG1 Isotype Control Cat. No.560373; Right Panel Two-color flow cytometric dot plots showing the correlated expression patterns of CD8 and Granzyme B or Mouse IgG1 Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 and either BD Horizon V450 Mouse Anti-Human Granzyme B antibody Cat. No.561151; Left Panel or BD Horizon V450 Mouse IgG1 Isotype Control Cat. No.560373; Right Panel Two-color flow cytometric dot plots showing the correlated expression patterns of CD8 and Granzyme B or Mouse IgG1 Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 and either BD Horizon V450 Mouse Anti-Human Granzyme B antibody Cat. No.561151; Left Panel or BD Horizon V450 Mouse IgG1 Isotype Control Cat. No.560373; Right Panel Two-color flow cytometric dot plots showing the correlated expression patterns of CD8 and Granzyme B or Mouse IgG1 Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 and either BD Horizon V450 Mouse Anti-Human Granzyme B antibody Cat. No.561151; Left Panel or BD Horizon V450 Mouse IgG1 Isotype Control Cat. No.560373; Right Panel Two-color flow cytometric dot plots showing the correlated expression patterns of CD8 and Granzyme B or Mouse IgG1 Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of Granzyme B expression by peripheral blood lymphocytes. Human PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 647 Mouse Anti-Human CD8 Cat. No. 557708 and either BD Horizon V450 Mouse Anti-Human Granzyme B antibody Cat. No.561151; Left Panel or BD Horizon V450 Mouse IgG1 Isotype Control Cat. No.560373; Right Panel Two-color flow cytometric dot plots showing the correlated expression patterns of CD8 and Granzyme B or Mouse IgG1 Isotype Control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.