BD HorizonTM V450 Mouse Anti-Human IFN-alpha[2b]

Description

Multicolor flow cytometric analysis of IFN-alpha2b in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated using CpG oligodeoxynucleotide Coley Pharmaceutical Co., Cat. No. 2336 and then treated with BD FastImmune Brefeldin A BFA Solution Cat. No. 347688 . Cells were harvested, washed, and stained with FITC-conjugated Lin-1 Cocktail Cat. No. 340546 , PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714/560904 , and APC Mouse Anti-Human HLA-DR Cat. No. 559868 simultaneously. The cells were then fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with BD Horizon V450 Mouse Anti-Human IFN-alpha2b IFN-alpha2b; Cat. No. 561382 . The two-color flow cytometric dot plots show the expression of CD123 versus IFN-alpha Right Panel derived from gated events with the light scattering characteristics of lymphocytes and monocytes and the immunofluorescence characteristics of Lin-1 negative and HLA-DR positive cells Left Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of IFN-alpha2b in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated using CpG oligodeoxynucleotide Coley Pharmaceutical Co., Cat. No. 2336 and then treated with BD FastImmune Brefeldin A BFA Solution Cat. No. 347688 . Cells were harvested, washed, and stained with FITC-conjugated Lin-1 Cocktail Cat. No. 340546 , PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714/560904 , and APC Mouse Anti-Human HLA-DR Cat. No. 559868 simultaneously. The cells were then fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with BD Horizon V450 Mouse Anti-Human IFN-alpha2b IFN-alpha2b; Cat. No. 561382 . The two-color flow cytometric dot plots show the expression of CD123 versus IFN-alpha Right Panel derived from gated events with the light scattering characteristics of lymphocytes and monocytes and the immunofluorescence characteristics of Lin-1 negative and HLA-DR positive cells Left Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of IFN-alpha2b in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated using CpG oligodeoxynucleotide Coley Pharmaceutical Co., Cat. No. 2336 and then treated with BD FastImmune Brefeldin A BFA Solution Cat. No. 347688 . Cells were harvested, washed, and stained with FITC-conjugated Lin-1 Cocktail Cat. No. 340546 , PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714/560904 , and APC Mouse Anti-Human HLA-DR Cat. No. 559868 simultaneously. The cells were then fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with BD Horizon V450 Mouse Anti-Human IFN-alpha2b IFN-alpha2b; Cat. No. 561382 . The two-color flow cytometric dot plots show the expression of CD123 versus IFN-alpha Right Panel derived from gated events with the light scattering characteristics of lymphocytes and monocytes and the immunofluorescence characteristics of Lin-1 negative and HLA-DR positive cells Left Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of IFN-alpha2b in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated using CpG oligodeoxynucleotide Coley Pharmaceutical Co., Cat. No. 2336 and then treated with BD FastImmune Brefeldin A BFA Solution Cat. No. 347688 . Cells were harvested, washed, and stained with FITC-conjugated Lin-1 Cocktail Cat. No. 340546 , PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714/560904 , and APC Mouse Anti-Human HLA-DR Cat. No. 559868 simultaneously. The cells were then fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with BD Horizon V450 Mouse Anti-Human IFN-alpha2b IFN-alpha2b; Cat. No. 561382 . The two-color flow cytometric dot plots show the expression of CD123 versus IFN-alpha Right Panel derived from gated events with the light scattering characteristics of lymphocytes and monocytes and the immunofluorescence characteristics of Lin-1 negative and HLA-DR positive cells Left Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of IFN-alpha2b in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated using CpG oligodeoxynucleotide Coley Pharmaceutical Co., Cat. No. 2336 and then treated with BD FastImmune Brefeldin A BFA Solution Cat. No. 347688 . Cells were harvested, washed, and stained with FITC-conjugated Lin-1 Cocktail Cat. No. 340546 , PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714/560904 , and APC Mouse Anti-Human HLA-DR Cat. No. 559868 simultaneously. The cells were then fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with BD Horizon V450 Mouse Anti-Human IFN-alpha2b IFN-alpha2b; Cat. No. 561382 . The two-color flow cytometric dot plots show the expression of CD123 versus IFN-alpha Right Panel derived from gated events with the light scattering characteristics of lymphocytes and monocytes and the immunofluorescence characteristics of Lin-1 negative and HLA-DR positive cells Left Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of IFN-alpha2b in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated using CpG oligodeoxynucleotide Coley Pharmaceutical Co., Cat. No. 2336 and then treated with BD FastImmune Brefeldin A BFA Solution Cat. No. 347688 . Cells were harvested, washed, and stained with FITC-conjugated Lin-1 Cocktail Cat. No. 340546 , PerCP-Cy5.5 Mouse Anti-Human CD123 Cat. No. 558714/560904 , and APC Mouse Anti-Human HLA-DR Cat. No. 559868 simultaneously. The cells were then fixed and permeabilized see Recommended Assay Procedure followed by intracellular staining with BD Horizon V450 Mouse Anti-Human IFN-alpha2b IFN-alpha2b; Cat. No. 561382 . The two-color flow cytometric dot plots show the expression of CD123 versus IFN-alpha Right Panel derived from gated events with the light scattering characteristics of lymphocytes and monocytes and the immunofluorescence characteristics of Lin-1 negative and HLA-DR positive cells Left Panel . Flow cytometry was performed using a BD LSR II Flow Cytometer System.