BD HorizonTM V450 Mouse Anti-Human IFN-gamma

Description

Expression of IFN-gamma by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated with PMA, Ionomycin in the presence of protein transport inhibitor Monensin GolgiStop Cat. No. 554724 . Cells were harvested, fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 , and stained with APC Mouse Anti-Human CD8 Cat. No. 555369 , PE Mouse Anti-Human CD3 Cat. No. 555333 and either BD Horizon V450 Mouse Anti-Human IFN-gamma Cat. No. 560372; left panel or BD Horizon V450 Mouse IgG1, kappa Isotype Control Cat. No. 560373, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes, then gated on CD3 positive events. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed on a BD LSR II flow cytometry system. Expression of IFN-gamma by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated with PMA, Ionomycin in the presence of protein transport inhibitor Monensin GolgiStop Cat. No. 554724 . Cells were harvested, fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 , and stained with APC Mouse Anti-Human CD8 Cat. No. 555369 , PE Mouse Anti-Human CD3 Cat. No. 555333 and either BD Horizon V450 Mouse Anti-Human IFN-gamma Cat. No. 560372; left panel or BD Horizon V450 Mouse IgG1, kappa Isotype Control Cat. No. 560373, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes, then gated on CD3 positive events. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed on a BD LSR II flow cytometry system. Expression of IFN-gamma by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated with PMA, Ionomycin in the presence of protein transport inhibitor Monensin GolgiStop Cat. No. 554724 . Cells were harvested, fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 , and stained with APC Mouse Anti-Human CD8 Cat. No. 555369 , PE Mouse Anti-Human CD3 Cat. No. 555333 and either BD Horizon V450 Mouse Anti-Human IFN-gamma Cat. No. 560372; left panel or BD Horizon V450 Mouse IgG1, kappa Isotype Control Cat. No. 560373, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes, then gated on CD3 positive events. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Expression of IFN-gamma by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated with PMA, Ionomycin in the presence of protein transport inhibitor Monensin GolgiStop Cat. No. 554724 . Cells were harvested, fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 , and stained with APC Mouse Anti-Human CD8 Cat. No. 555369 , PE Mouse Anti-Human CD3 Cat. No. 555333 and either BD Horizon V450 Mouse Anti-Human IFN-gamma Cat. No. 560372; left panel or BD Horizon V450 Mouse IgG1, kappa Isotype Control Cat. No. 560373, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes, then gated on CD3 positive events. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Expression of IFN-gamma by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated with PMA, Ionomycin in the presence of protein transport inhibitor Monensin GolgiStop Cat. No. 554724 . Cells were harvested, fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 , and stained with APC Mouse Anti-Human CD8 Cat. No. 555369 , PE Mouse Anti-Human CD3 Cat. No. 555333 and either BD Horizon V450 Mouse Anti-Human IFN-gamma Cat. No. 560372; left panel or BD Horizon V450 Mouse IgG1, kappa Isotype Control Cat. No. 560373, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes, then gated on CD3 positive events. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed on a BD LSR II flow cytometry system. Expression of IFN-gamma by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated with PMA, Ionomycin in the presence of protein transport inhibitor Monensin GolgiStop Cat. No. 554724 . Cells were harvested, fixed and permeabilized using the BD Cytofix/Cytoperm Kit Cat. No. 554714 , and stained with APC Mouse Anti-Human CD8 Cat. No. 555369 , PE Mouse Anti-Human CD3 Cat. No. 555333 and either BD Horizon V450 Mouse Anti-Human IFN-gamma Cat. No. 560372; left panel or BD Horizon V450 Mouse IgG1, kappa Isotype Control Cat. No. 560373, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes, then gated on CD3 positive events. The quadrant markers for the bivariate dot plots were set based on the isotype controls. Flow cytometry was performed on a BD LSR II flow cytometry system.