BD HorizonTM V450 Mouse anti-Nestin

Description

Analysis of Nestin staining on H9 derived Neural Stem Cells NSC, left and Neurons right . NSC and neurons were derived from H9 human embryonic stem cells WiCell, Madison, WI . The NSC and neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm/Wash buffer III Cat. No. 558050 . The left panel shows NSC that were stained with matching concentrations of either BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No.560373 or V450 Mouse anti-Nestin monoclonal antibody solid line, Cat. No. 561551 . The right panel shows neurons that were stained with V450 Mouse anti-Nestin and PerCP-Cy5.5 Mouse anti-Sox2 Cat. No. 561506 monoclonal antibodies. The histograms were derived from gated events based on the light scattering characteristics of NSC and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. The neurons were derived from a sorted population of H9-derived NSC that were then differentiated for 4 weeks in NSC differentiation medium containing N2/B27 supplement Life Technologies , BDNF, GDNF Peprotech , and dibutryl cyclic-AMP Sigma . The double-positive population consists of NSC and glial cells, while the double-negative population consists primarily of neurons. Analysis of Nestin staining on H9 derived Neural Stem Cells NSC, left and Neurons right . NSC and neurons were derived from H9 human embryonic stem cells WiCell, Madison, WI . The NSC and neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm/Wash buffer III Cat. No. 558050 . The left panel shows NSC that were stained with matching concentrations of either BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No.560373 or V450 Mouse anti-Nestin monoclonal antibody solid line, Cat. No. 561551 . The right panel shows neurons that were stained with V450 Mouse anti-Nestin and PerCP-Cy5.5 Mouse anti-Sox2 Cat. No. 561506 monoclonal antibodies. The histograms were derived from gated events based on the light scattering characteristics of NSC and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. The neurons were derived from a sorted population of H9-derived NSC that were then differentiated for 4 weeks in NSC differentiation medium containing N2/B27 supplement Life Technologies , BDNF, GDNF Peprotech , and dibutryl cyclic-AMP Sigma . The double-positive population consists of NSC and glial cells, while the double-negative population consists primarily of neurons. Analysis of Nestin staining on H9 derived Neural Stem Cells NSC, left and Neurons right . NSC and neurons were derived from H9 human embryonic stem cells WiCell, Madison, WI . The NSC and neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm/Wash buffer III Cat. No. 558050 . The left panel shows NSC that were stained with matching concentrations of either BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No.560373 or V450 Mouse anti-Nestin monoclonal antibody solid line, Cat. No. 561551 . The right panel shows neurons that were stained with V450 Mouse anti-Nestin and PerCP-Cy5.5 Mouse anti-Sox2 Cat. No. 561506 monoclonal antibodies. The histograms were derived from gated events based on the light scattering characteristics of NSC and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. The neurons were derived from a sorted population of H9-derived NSC that were then differentiated for 4 weeks in NSC differentiation medium containing N2/B27 supplement Life Technologies , BDNF, GDNF Peprotech , and dibutryl cyclic-AMP Sigma . The double-positive population consists of NSC and glial cells, while the double-negative population consists primarily of neurons. Show More Analysis of Nestin staining on H9 derived Neural Stem Cells NSC, left and Neurons right . NSC and neurons were derived from H9 human embryonic stem cells WiCell, Madison, WI . The NSC and neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm/Wash buffer III Cat. No. 558050 . The left panel shows NSC that were stained with matching concentrations of either BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No.560373 or V450 Mouse anti-Nestin monoclonal antibody solid line, Cat. No. 561551 . The right panel shows neurons that were stained with V450 Mouse anti-Nestin and PerCP-Cy5.5 Mouse anti-Sox2 Cat. No. 561506 monoclonal antibodies. The histograms were derived from gated events based on the light scattering characteristics of NSC and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. The neurons were derived from a sorted population of H9-derived NSC that were then differentiated for 4 weeks in NSC differentiation medium containing N2/B27 supplement Life Technologies , BDNF, GDNF Peprotech , and dibutryl cyclic-AMP Sigma . The double-positive population consists of NSC and glial cells, while the double-negative population consists primarily of neurons. Show More Analysis of Nestin staining on H9 derived Neural Stem Cells NSC, left and Neurons right . NSC and neurons were derived from H9 human embryonic stem cells WiCell, Madison, WI . The NSC and neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm/Wash buffer III Cat. No. 558050 . The left panel shows NSC that were stained with matching concentrations of either BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No.560373 or V450 Mouse anti-Nestin monoclonal antibody solid line, Cat. No. 561551 . The right panel shows neurons that were stained with V450 Mouse anti-Nestin and PerCP-Cy5.5 Mouse anti-Sox2 Cat. No. 561506 monoclonal antibodies. The histograms were derived from gated events based on the light scattering characteristics of NSC and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. The neurons were derived from a sorted population of H9-derived NSC that were then differentiated for 4 weeks in NSC differentiation medium containing N2/B27 supplement Life Technologies , BDNF, GDNF Peprotech , and dibutryl cyclic-AMP Sigma . The double-positive population consists of NSC and glial cells, while the double-negative population consists primarily of neurons. Analysis of Nestin staining on H9 derived Neural Stem Cells NSC, left and Neurons right . NSC and neurons were derived from H9 human embryonic stem cells WiCell, Madison, WI . The NSC and neurons were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm/Wash buffer III Cat. No. 558050 . The left panel shows NSC that were stained with matching concentrations of either BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No.560373 or V450 Mouse anti-Nestin monoclonal antibody solid line, Cat. No. 561551 . The right panel shows neurons that were stained with V450 Mouse anti-Nestin and PerCP-Cy5.5 Mouse anti-Sox2 Cat. No. 561506 monoclonal antibodies. The histograms were derived from gated events based on the light scattering characteristics of NSC and neurons, respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. The neurons were derived from a sorted population of H9-derived NSC that were then differentiated for 4 weeks in NSC differentiation medium containing N2/B27 supplement Life Technologies , BDNF, GDNF Peprotech , and dibutryl cyclic-AMP Sigma . The double-positive population consists of NSC and glial cells, while the double-negative population consists primarily of neurons.