BD HorizonTM V450 Mouse anti-Sox2

Description

Analysis of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No. 560373 or BD Horizon V450 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics for the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate. Analysis of Sox2 on human ES cell-derived neural stem cells NSC . NSC derived from H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No. 560373 or BD Horizon V450 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics for the H9-derived NSC. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate. Analysis of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No. 560373 or BD Horizon V450 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics for the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate. Show More Analysis of Sox2 on human ES cell-derived neural stem cells NSC . NSC derived from H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No. 560373 or BD Horizon V450 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics for the H9-derived NSC. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate. Show More Analysis of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of a BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No. 560373 or BD Horizon V450 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics for the H9 cell line. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate. Analysis of Sox2 on human ES cell-derived neural stem cells NSC . NSC derived from H9 human ES cells WiCell, Madison, WI were harvested, fixed in BD Cytofix buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash buffer I Cat. No. 557885 and stained with matching concentrations of BD Horizon V450 Mouse IgG1, kappa isotype control dashed line, Cat. No. 560373 or BD Horizon V450 Mouse Anti-Sox2 monoclonal antibody solid line . Histograms were derived from gated events based on light scattering characteristics for the H9-derived NSC. Flow cytometry was performed on a BD LSR II flow cytometry system. BD Phosflow Perm Buffer III can also be used with this antibody conjugate.