BD IMagTM Anti-CD11b Magnetic Particles - DM

Description

Positive selection and depletion of mouse CD11b-positive lymphocytes. Bone marrow cells were labeled with BD IMag anti-CD11b Particles - DM Cat. No. 558013 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD11b- and positive CD11b+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh bone marrow left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC Rat Anti-CD11b Cat. No. 553310 and PE Rat Anti-mouse Ly-6G and Ly-6C RB6-8C5 Cat. No. 553128 . The percent CD11b+ cells in each smple is given. The expected cell recovery ranges from 60-80 %. Positive selection and depletion of mouse CD11b-positive lymphocytes. Bone marrow cells were labeled with BD IMag anti-CD11b Particles - DM Cat. No. 558013 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD11b- and positive CD11b+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh bone marrow left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC Rat Anti-CD11b Cat. No. 553310 and PE Rat Anti-mouse Ly-6G and Ly-6C RB6-8C5 Cat. No. 553128 . The percent CD11b+ cells in each smple is given. The expected cell recovery ranges from 60-80 %. Show More Positive selection and depletion of mouse CD11b-positive lymphocytes. Bone marrow cells were labeled with BD IMag anti-CD11b Particles - DM Cat. No. 558013 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD11b- and positive CD11b+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh bone marrow left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC Rat Anti-CD11b Cat. No. 553310 and PE Rat Anti-mouse Ly-6G and Ly-6C RB6-8C5 Cat. No. 553128 . The percent CD11b+ cells in each smple is given. The expected cell recovery ranges from 60-80 %.