BD IMagTM Anti-Human CD25 Magnetic Particles - DM

Description

Depletion and positive selection of CD25-positive T lymphocytes. Freshly isolated far and middle left panels or anti-CD3-stimulated far and middle right panels PBMC were labeled with BD IMag Anti-Human CD25 Magnetic Particles - DM Cat. No. 558005 as described in the protocol. After labeling, the cell were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD25- or positive CD25+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, unseparated PBMC far left panel and their negative fraction middle left panel or unseparated CD3-activated PBMC middle right panel and their positive fraction far right panel were stained with APC Mouse Anti-Human CD3 Cat. No. 555335 and FITC Mouse Anti-Human CD25 Cat. No. 555431 . Nonviable cells were excluded by staining with Propidium Iodide Staining Solution Cat. No.556463 , and the viable CD3+ leukocytes are displayed. The percentage of the CD3+ cells which are CD25+ in each panel is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry instrument. Depletion and positive selection of CD25-positive T lymphocytes. Freshly isolated far and middle left panels or anti-CD3-stimulated far and middle right panels PBMC were labeled with BD IMag Anti-Human CD25 Magnetic Particles - DM Cat. No. 558005 as described in the protocol. After labeling, the cell were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD25- or positive CD25+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, unseparated PBMC far left panel and their negative fraction middle left panel or unseparated CD3-activated PBMC middle right panel and their positive fraction far right panel were stained with APC Mouse Anti-Human CD3 Cat. No. 555335 and FITC Mouse Anti-Human CD25 Cat. No. 555431 . Nonviable cells were excluded by staining with Propidium Iodide Staining Solution Cat. No.556463 , and the viable CD3+ leukocytes are displayed. The percentage of the CD3+ cells which are CD25+ in each panel is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry instrument. Show More Depletion and positive selection of CD25-positive T lymphocytes. Freshly isolated far and middle left panels or anti-CD3-stimulated far and middle right panels PBMC were labeled with BD IMag Anti-Human CD25 Magnetic Particles - DM Cat. No. 558005 as described in the protocol. After labeling, the cell were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative CD25- or positive CD25+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, unseparated PBMC far left panel and their negative fraction middle left panel or unseparated CD3-activated PBMC middle right panel and their positive fraction far right panel were stained with APC Mouse Anti-Human CD3 Cat. No. 555335 and FITC Mouse Anti-Human CD25 Cat. No. 555431 . Nonviable cells were excluded by staining with Propidium Iodide Staining Solution Cat. No.556463 , and the viable CD3+ leukocytes are displayed. The percentage of the CD3+ cells which are CD25+ in each panel is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry instrument.