BD IMagTM Anti-Mouse IgG1 Magnetic Particles - DM

Description

Figure 1. Depletion of human T and B lymphocytes and NK cells. Human PBMC were stained with APC Mouse Anti-Human CD4 mAb RPA-T4 Cat. No. 555349 , CD8 mAb RPA-T8 Cat. No. 555369 , CD19 mAb HIB19 Cat. No. 555415 , and CD56 mAb B159 Cat. No. 555518 , then labeled with BD IMag Anti-Mouse IgG1 Magnetic Particles - DM Cat. No. 557983 . After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the depleted and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. Please refer to the Depletion Flow Chart to identify the separated cell populations represented in this figure. Unseparated PBMC left panel , the final depleted fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with Propidium Iodide Staining Solution Cat. No. 557983 , and mononuclear cells were identified by scatter profile. The percentage of non-T-B-NK leukocytes in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Figure 2. Positive selection of rat alpha TCR-expressing T lymphocytes. Lewis rat splenocytes were stained with FITC Mouse Anti-Rat alpha TCR mAb R73 Cat. No. 554913 , and then labeled with BD IMag Anti-Mouse IgG1 Magnetic Particles - DM. After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the negative and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. Unseparated Spleen left panel , the Negative Fraction middle panel , and the Positive Fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with propidium iodide, and all viable leukocytes are displayed. The percentage of alphabeta TCR+ T lymphocytes in each sample i Figure 1. Depletion of human T and B lymphocytes and NK cells. Human PBMC were stained with APC Mouse Anti-Human CD4 mAb RPA-T4 Cat. No. 555349 , CD8 mAb RPA-T8 Cat. No. 555369 , CD19 mAb HIB19 Cat. No. 555415 , and CD56 mAb B159 Cat. No. 555518 , then labeled with BD IMag Anti-Mouse IgG1 Magnetic Particles - DM Cat. No. 557983 . After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the depleted and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. Please refer to the Depletion Flow Chart to identify the separated cell populations represented in this figure. Unseparated PBMC left panel , the final depleted fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with Propidium Iodide Staining Solution Cat. No. 557983 , and mononuclear cells were identified by scatter profile. The percentage of non-T-B-NK leukocytes in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Figure 2. Positive selection of rat alpha TCR-expressing T lymphocytes. Lewis rat splenocytes were stained with FITC Mouse Anti-Rat alpha TCR mAb R73 Cat. No. 554913 , and then labeled with BD IMag Anti-Mouse IgG1 Magnetic Particles - DM. After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the negative and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. Unseparated Spleen left panel , the Negative Fraction middle panel , and the Positive Fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with propidium iodide, and all viable leukocytes are displayed. The percentage of alphabeta TCR+ T lymphocytes in each sample i Show More Figure 1. Depletion of human T and B lymphocytes and NK cells. Human PBMC were stained with APC Mouse Anti-Human CD4 mAb RPA-T4 Cat. No. 555349 , CD8 mAb RPA-T8 Cat. No. 555369 , CD19 mAb HIB19 Cat. No. 555415 , and CD56 mAb B159 Cat. No. 555518 , then labeled with BD IMag Anti-Mouse IgG1 Magnetic Particles - DM Cat. No. 557983 . After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the depleted and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. Please refer to the Depletion Flow Chart to identify the separated cell populations represented in this figure. Unseparated PBMC left panel , the final depleted fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with Propidium Iodide Staining Solution Cat. No. 557983 , and mononuclear cells were identified by scatter profile. The percentage of non-T-B-NK leukocytes in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Figure 2. Positive selection of rat alpha TCR-expressing T lymphocytes. Lewis rat splenocytes were stained with FITC Mouse Anti-Rat alpha TCR mAb R73 Cat. No. 554913 , and then labeled with BD IMag Anti-Mouse IgG1 Magnetic Particles - DM. After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the negative and positive fractions were collected as described in the Magnetic Labeling and Separation Protocol. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. Unseparated Spleen left panel , the Negative Fraction middle panel , and the Positive Fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with propidium iodide, and all viable leukocytes are displayed. The percentage of alphabeta TCR+ T lymphocytes in each sample i