BD IMagTM Anti-Mouse Ly-6G and Ly-6C Particles - DM

Description

Positive selection and depletion of mouse Ly-6g and Ly-6C Gr-1 positive lymphocytes. Bone marrow cells were labeled with BD IMag anti-mouse Ly-6G and Ly-6C Gr-1 Particles - DM Cat. No. 558111 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative Ly-6G and Ly-6g- and positive Ly-6G and Ly-6C+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh bone marrow left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC-conjugated anti-mouse CD11b mAb M1/70 Cat. No. 553310 and PE-conjugated anti-mouse Ly-6G and Ly-6C Gr-1 mAb RB6-8C5 Cat. No. 553128 . The percent Gr-1+ cells in each sample is given. The expected cell recovery ranges from 60 % to 80 %. Positive selection and depletion of mouse Ly-6g and Ly-6C Gr-1 positive lymphocytes. Bone marrow cells were labeled with BD IMag anti-mouse Ly-6G and Ly-6C Gr-1 Particles - DM Cat. No. 558111 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative Ly-6G and Ly-6g- and positive Ly-6G and Ly-6C+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh bone marrow left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC-conjugated anti-mouse CD11b mAb M1/70 Cat. No. 553310 and PE-conjugated anti-mouse Ly-6G and Ly-6C Gr-1 mAb RB6-8C5 Cat. No. 553128 . The percent Gr-1+ cells in each sample is given. The expected cell recovery ranges from 60 % to 80 %. Show More Positive selection and depletion of mouse Ly-6g and Ly-6C Gr-1 positive lymphocytes. Bone marrow cells were labeled with BD IMag anti-mouse Ly-6G and Ly-6C Gr-1 Particles - DM Cat. No. 558111 as described in the protocol. After labeling, the cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 , and the negative Ly-6G and Ly-6g- and positive Ly-6G and Ly-6C+ fractions were collected. Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh bone marrow left panel , the negative fraction middle panel , and the positive fraction right panel were stained with FITC-conjugated anti-mouse CD11b mAb M1/70 Cat. No. 553310 and PE-conjugated anti-mouse Ly-6G and Ly-6C Gr-1 mAb RB6-8C5 Cat. No. 553128 . The percent Gr-1+ cells in each sample is given. The expected cell recovery ranges from 60 % to 80 %.