BD IMagTM APC Magnetic Particles - DM

Description

Depletion of T-, B-, NK-, and myeloid-lineage cells from mouse bone marrow top panels . BALB/c bone-marrow cells were labeled with APC Anti-Mouse CD3e Cat. No. 553066 , CD11b Cat. No. 553312 , CD45R/B220 Cat. No. 553092 , and Ly-6G and Ly-6C Cat. No. 553129 followed by BD IMag Anti-APC Particles - DM Cat. No.557932 according to the depletion protocol. The labeled cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 . Please refer to the Depletion Flow Chart to identify the separated cell populations represented in this figure. Unseparated bone-marrow cells left panel , the final depleted fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. The percentage of positive cells is indicated in each panel; placement of each marker is based upon staining with the appropriate isotype control data not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Positive selection of human B lymphocytes bottom panels . Peripheral blood mononuclear cells PBMC were stained with APC Anti-Human CD19 Cat. No. 555415 and FITC Anti-Human CD45RA Cat. No. 555488 , and then labeled with BD IMag Anti-APC Particles - DM. After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the negative CD19- and positive CD19+ fractions were collected as described in the positive selection protocol. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. Unseparated PBMC left panel , the negative fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with Propidium Iodide Staining Solution Cat. No. 556463 , and all viable leukocytes are displayed. The percentage of CD19+ B lymphocytes in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Optimal concentrations of BD IMag Anti-APC Particles - DM for positive selection with some APC-conjugated monoclonal antibodies to human and mouse leukocyte antigens. Depletion of T-, B-, NK-, and myeloid-lineage cells from mouse bone marrow top panels . BALB/c bone-marrow cells were labeled with APC Anti-Mouse CD3e Cat. No. 553066 , CD11b Cat. No. 553312 , CD45R/B220 Cat. No. 553092 , and Ly-6G and Ly-6C Cat. No. 553129 followed by BD IMag Anti-APC Particles - DM Cat. No.557932 according to the depletion protocol. The labeled cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 . Please refer to the Depletion Flow Chart to identify the separated cell populations represented in this figure. Unseparated bone-marrow cells left panel , the final depleted fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. The percentage of positive cells is indicated in each panel; placement of each marker is based upon staining with the appropriate isotype control data not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Positive selection of human B lymphocytes bottom panels . Peripheral blood mononuclear cells PBMC were stained with APC Anti-Human CD19 Cat. No. 555415 and FITC Anti-Human CD45RA Cat. No. 555488 , and then labeled with BD IMag Anti-APC Particles - DM. After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the negative CD19- and positive CD19+ fractions were collected as described in the positive selection protocol. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. Unseparated PBMC left panel , the negative fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with Propidium Iodide Staining Solution Cat. No. 556463 , and all viable leukocytes are displayed. The percentage of CD19+ B lymphocytes in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Optimal concentrations of BD IMag Anti-APC Particles - DM for positive selection with some APC-conjugated monoclonal antibodies to human and mouse leukocyte antigens. Show More Depletion of T-, B-, NK-, and myeloid-lineage cells from mouse bone marrow top panels . BALB/c bone-marrow cells were labeled with APC Anti-Mouse CD3e Cat. No. 553066 , CD11b Cat. No. 553312 , CD45R/B220 Cat. No. 553092 , and Ly-6G and Ly-6C Cat. No. 553129 followed by BD IMag Anti-APC Particles - DM Cat. No.557932 according to the depletion protocol. The labeled cells were separated using the BD IMag Cell Separation Magnet Cat. No. 552311 . Please refer to the Depletion Flow Chart to identify the separated cell populations represented in this figure. Unseparated bone-marrow cells left panel , the final depleted fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. The percentage of positive cells is indicated in each panel; placement of each marker is based upon staining with the appropriate isotype control data not shown . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Positive selection of human B lymphocytes bottom panels . Peripheral blood mononuclear cells PBMC were stained with APC Anti-Human CD19 Cat. No. 555415 and FITC Anti-Human CD45RA Cat. No. 555488 , and then labeled with BD IMag Anti-APC Particles - DM. After labeling, the cells were separated using the BD IMag Cell Separation Magnet, and the negative CD19- and positive CD19+ fractions were collected as described in the positive selection protocol. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. Unseparated PBMC left panel , the negative fraction middle panel , and the positive fraction right panel were analyzed by flow cytometry. Nonviable cells were eliminated from analysis by staining with Propidium Iodide Staining Solution Cat. No. 556463 , and all viable leukocytes are displayed. The percentage of CD19+ B lymphocytes in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Optimal concentrations of BD IMag Anti-APC Particles - DM for positive selection with some APC-conjugated monoclonal antibodies to human and mouse leukocyte antigens.