BD IMagTM Human CD8 T Lymphocyte Enrichment Set-DM

Description

Enrichment of CD8 T lymphocytes from human blood. PBMC were labeled with the BD IMag Human CD8 T Lymphocyte Enrichment Set - DM and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. Cells were stained with APC Mouse Anti-Human CD8 Cat. No. 555369 to detect CD8 T lymphocytes and a mixture of PE Mouse Anti-Human CD4 Cat. No. 555347 , CD11b/ Mac-1 Cat. No. 555388 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD36 Cat. No. 555455 , CD56 Cat. No. 555516 , CD123 Cat. No. 554529 , CD235a Cat. No. 555570 , and gammadelta TCR Cat. No. 555717 monoclonal antibodies to detect non-CD8 T leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD8 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional separation step can result in 5-10 % increased purity with less than a 5 % decrease in recovery. Far right panel shows the positive fraction. Enrichment of CD8 T lymphocytes from human blood. PBMC were labeled with the BD IMag Human CD8 T Lymphocyte Enrichment Set - DM and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. Cells were stained with APC Mouse Anti-Human CD8 Cat. No. 555369 to detect CD8 T lymphocytes and a mixture of PE Mouse Anti-Human CD4 Cat. No. 555347 , CD11b/ Mac-1 Cat. No. 555388 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD36 Cat. No. 555455 , CD56 Cat. No. 555516 , CD123 Cat. No. 554529 , CD235a Cat. No. 555570 , and gammadelta TCR Cat. No. 555717 monoclonal antibodies to detect non-CD8 T leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD8 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional separation step can result in 5-10 % increased purity with less than a 5 % decrease in recovery. Far right panel shows the positive fraction. Show More Enrichment of CD8 T lymphocytes from human blood. PBMC were labeled with the BD IMag Human CD8 T Lymphocyte Enrichment Set - DM and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. Cells were stained with APC Mouse Anti-Human CD8 Cat. No. 555369 to detect CD8 T lymphocytes and a mixture of PE Mouse Anti-Human CD4 Cat. No. 555347 , CD11b/ Mac-1 Cat. No. 555388 , CD16 Cat. No. 555407 , CD19 Cat. No. 555413 , CD36 Cat. No. 555455 , CD56 Cat. No. 555516 , CD123 Cat. No. 554529 , CD235a Cat. No. 555570 , and gammadelta TCR Cat. No. 555717 monoclonal antibodies to detect non-CD8 T leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD8 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional separation step can result in 5-10 % increased purity with less than a 5 % decrease in recovery. Far right panel shows the positive fraction.