BD IMagTM Human Dendritic Cell Enrichment Set - DM

Description

Enrichment of dendritic cells from human blood. PBMC were labeled with the BD IMag Human dendritic cell Enrichment Set - DM Cat. No. 558420 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a lineage cocktail consisting of APC Mouse Anti-Human CD3 Cat. No. 555335 , CD19 Cat. No. 555415 , CD14 Cat. No. 555399 , and CD56 Cat. No. 555518 and a dendritic cell cocktail consisting of PE Mouse Anti-Human CD11c Cat. No. 555392 , CD123 Cat. No. 555644 , CD16 Cat. No. 555407 and CD34 Cat. No. 550761 . Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of dendritic cells is indicated in the upper-left corner of each panel. Panel A shows unseparated PBMC. Panel B shows the twice-enriched fraction after three 6-minute magnetic separations with an additional 10-minute separation. Panel C shows the positive fraction. Enrichment of dendritic cells from human blood. PBMC were labeled with the BD IMag Human dendritic cell Enrichment Set - DM Cat. No. 558420 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a lineage cocktail consisting of APC Mouse Anti-Human CD3 Cat. No. 555335 , CD19 Cat. No. 555415 , CD14 Cat. No. 555399 , and CD56 Cat. No. 555518 and a dendritic cell cocktail consisting of PE Mouse Anti-Human CD11c Cat. No. 555392 , CD123 Cat. No. 555644 , CD16 Cat. No. 555407 and CD34 Cat. No. 550761 . Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of dendritic cells is indicated in the upper-left corner of each panel. Panel A shows unseparated PBMC. Panel B shows the twice-enriched fraction after three 6-minute magnetic separations with an additional 10-minute separation. Panel C shows the positive fraction. Show More Enrichment of dendritic cells from human blood. PBMC were labeled with the BD IMag Human dendritic cell Enrichment Set - DM Cat. No. 558420 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with a lineage cocktail consisting of APC Mouse Anti-Human CD3 Cat. No. 555335 , CD19 Cat. No. 555415 , CD14 Cat. No. 555399 , and CD56 Cat. No. 555518 and a dendritic cell cocktail consisting of PE Mouse Anti-Human CD11c Cat. No. 555392 , CD123 Cat. No. 555644 , CD16 Cat. No. 555407 and CD34 Cat. No. 550761 . Dead cells were excluded by staining with 7-Amino-actinomycin D 7-AAD Cat. No. 559925 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of dendritic cells is indicated in the upper-left corner of each panel. Panel A shows unseparated PBMC. Panel B shows the twice-enriched fraction after three 6-minute magnetic separations with an additional 10-minute separation. Panel C shows the positive fraction.