BD IMagTM Human NK Cell Enrichment Set - DM

Description

Enrichment of NK cells from human blood. PBMC were labeled with the BD IMag Human NK Cell Enrichment Set - DM Cat. No. 557987 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with APC Anti-Human CD56 Cat. No. 555518 to detect NK cells and a mixture of PE Mouse Anti-Human CD3 Cat. No. 555333 , Anti-Human CD19 Cat. No. 555413 , Anti-Human CD36 Cat. No. 555455 , Anti-Human CD123 Cat. No. 555644 , and Anti-Human CD235a Cat. No. 555570 monoclonal antibodies to detect non-NK leukocytes including NK-T cells and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 , and leukocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of NK cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional separation step typically results in >5 % increased purity with less than a 5 % decrease in recovery. Far right panel shows the positive fraction. Enrichment of NK cells from human blood. PBMC were labeled with the BD IMag Human NK Cell Enrichment Set - DM Cat. No. 557987 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with APC Anti-Human CD56 Cat. No. 555518 to detect NK cells and a mixture of PE Mouse Anti-Human CD3 Cat. No. 555333 , Anti-Human CD19 Cat. No. 555413 , Anti-Human CD36 Cat. No. 555455 , Anti-Human CD123 Cat. No. 555644 , and Anti-Human CD235a Cat. No. 555570 monoclonal antibodies to detect non-NK leukocytes including NK-T cells and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 , and leukocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of NK cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional separation step typically results in >5 % increased purity with less than a 5 % decrease in recovery. Far right panel shows the positive fraction. Show More Enrichment of NK cells from human blood. PBMC were labeled with the BD IMag Human NK Cell Enrichment Set - DM Cat. No. 557987 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with APC Anti-Human CD56 Cat. No. 555518 to detect NK cells and a mixture of PE Mouse Anti-Human CD3 Cat. No. 555333 , Anti-Human CD19 Cat. No. 555413 , Anti-Human CD36 Cat. No. 555455 , Anti-Human CD123 Cat. No. 555644 , and Anti-Human CD235a Cat. No. 555570 monoclonal antibodies to detect non-NK leukocytes including NK-T cells and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 , and leukocytes were selected by scatter profile. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of NK cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated PBMC. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional separation step typically results in >5 % increased purity with less than a 5 % decrease in recovery. Far right panel shows the positive fraction.