BD IMagTM Mouse CD4 T Lymphocyte Enrichment Set - DM

Description

Enrichment of CD4 T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse CD4 T Lymphocyte Enrichment Set - DM Cat. No. 558131 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC Rat Anti-Mouse CD4 Cat. No. 557307/553729 to detect CD4 T lymphocytes and a mixture of PE Rat Anti-Mouse CD8a Cat. No. 553032/553033 , CD11b Cat. No. 557397/553311 , CD45R/B220 Cat. No. 553089/553090 , CD49b Cat. No. 558759 , and TER-119/Erythroid Cells Cat. No. 553673 monoclonal antibodies to detect CD4-negative leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD4 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated splenocytes. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. Far right panel shows the positive fraction. Enrichment of CD4 T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse CD4 T Lymphocyte Enrichment Set - DM Cat. No. 558131 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC Rat Anti-Mouse CD4 Cat. No. 557307/553729 to detect CD4 T lymphocytes and a mixture of PE Rat Anti-Mouse CD8a Cat. No. 553032/553033 , CD11b Cat. No. 557397/553311 , CD45R/B220 Cat. No. 553089/553090 , CD49b Cat. No. 558759 , and TER-119/Erythroid Cells Cat. No. 553673 monoclonal antibodies to detect CD4-negative leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD4 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated splenocytes. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. Far right panel shows the positive fraction. Show More Enrichment of CD4 T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse CD4 T Lymphocyte Enrichment Set - DM Cat. No. 558131 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC Rat Anti-Mouse CD4 Cat. No. 557307/553729 to detect CD4 T lymphocytes and a mixture of PE Rat Anti-Mouse CD8a Cat. No. 553032/553033 , CD11b Cat. No. 557397/553311 , CD45R/B220 Cat. No. 553089/553090 , CD49b Cat. No. 558759 , and TER-119/Erythroid Cells Cat. No. 553673 monoclonal antibodies to detect CD4-negative leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD4 T cells is indicated in the lower-right corner of each panel. Far left panel shows unseparated splenocytes. Middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. Far right panel shows the positive fraction.