BD IMagTM Mouse CD8 T Lymphocyte Enrichment Set - DM

Description

Enrichment of CD8 T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse CD8 T Lymphocyte Enrichment Set - DM Cat. No. 558471 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 and APC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553066 to detect CD8 T lymphocytes. Dead cells were excluded by staining with Propidium Iodide Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD8 T cells is indicated in the upper-right corner of each panel. Far left panel shows unseparated splenocytes. Middle left panel shows the combined fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. Far right panel shows the positive fraction. Enrichment of CD8 T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse CD8 T Lymphocyte Enrichment Set - DM Cat. No. 558471 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 and APC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553066 to detect CD8 T lymphocytes. Dead cells were excluded by staining with Propidium Iodide Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD8 T cells is indicated in the upper-right corner of each panel. Far left panel shows unseparated splenocytes. Middle left panel shows the combined fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. Far right panel shows the positive fraction. Show More Enrichment of CD8 T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse CD8 T Lymphocyte Enrichment Set - DM Cat. No. 558471 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC-conjugated anti-mouse CD8a mAb 53-6.7 Cat. No. 553030/553031 and APC-conjugated anti-mouse CD3e mAb 145-2C11 Cat. No. 553066 to detect CD8 T lymphocytes. Dead cells were excluded by staining with Propidium Iodide Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of CD8 T cells is indicated in the upper-right corner of each panel. Far left panel shows unseparated splenocytes. Middle left panel shows the combined fraction after three 6-minute magnetic separations. Middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. Far right panel shows the positive fraction.