BD IMagTM Mouse NK Cell Enrichment Set-DM

Description

Enrichment of NK cells from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse NK Cell Enrichment Set - DM Cat. No. 557954 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC-conjugated Anti-Mouse CD49b DX5 Cat. No. 553857 to detect NK cells and a mixture of PE-conjugated Anti-Mouse CD4 RM4-5 Cat. No. 553049 , Anti-Mouse CD5 53-7.3 Cat. No. 553023 , Anti-Mouse CD8a 53-6.7 Cat. No. 553032/553033 , Anti-Mouse CD19 1D3 Cat. No. 5553786 , Anti-Mouse CD24 M1/69 Cat. No. 553262 , Anti-Mouse Ly-6G and Ly-6C RB6-8C5 Cat. No. 553128 , and Anti-Mouse TER-119/Erythroid Cells TER-119 Cat. No. 553673 monoclonal antibodies to detect non-NK leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of NK cells is indicated in the lower-right corner of each panel. The left panel shows unseparated splenocytes. The middle panel shows the twice-enriched fraction after three 6-minute magnetic separations plus an additional 6-minute separation of those enriched cells. The right panel shows the positive fraction. Enrichment of NK cells from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse NK Cell Enrichment Set - DM Cat. No. 557954 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC-conjugated Anti-Mouse CD49b DX5 Cat. No. 553857 to detect NK cells and a mixture of PE-conjugated Anti-Mouse CD4 RM4-5 Cat. No. 553049 , Anti-Mouse CD5 53-7.3 Cat. No. 553023 , Anti-Mouse CD8a 53-6.7 Cat. No. 553032/553033 , Anti-Mouse CD19 1D3 Cat. No. 5553786 , Anti-Mouse CD24 M1/69 Cat. No. 553262 , Anti-Mouse Ly-6G and Ly-6C RB6-8C5 Cat. No. 553128 , and Anti-Mouse TER-119/Erythroid Cells TER-119 Cat. No. 553673 monoclonal antibodies to detect non-NK leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of NK cells is indicated in the lower-right corner of each panel. The left panel shows unseparated splenocytes. The middle panel shows the twice-enriched fraction after three 6-minute magnetic separations plus an additional 6-minute separation of those enriched cells. The right panel shows the positive fraction. Show More Enrichment of NK cells from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse NK Cell Enrichment Set - DM Cat. No. 557954 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC-conjugated Anti-Mouse CD49b DX5 Cat. No. 553857 to detect NK cells and a mixture of PE-conjugated Anti-Mouse CD4 RM4-5 Cat. No. 553049 , Anti-Mouse CD5 53-7.3 Cat. No. 553023 , Anti-Mouse CD8a 53-6.7 Cat. No. 553032/553033 , Anti-Mouse CD19 1D3 Cat. No. 5553786 , Anti-Mouse CD24 M1/69 Cat. No. 553262 , Anti-Mouse Ly-6G and Ly-6C RB6-8C5 Cat. No. 553128 , and Anti-Mouse TER-119/Erythroid Cells TER-119 Cat. No. 553673 monoclonal antibodies to detect non-NK leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart to identify the cell populations represented in this figure. The percentage of NK cells is indicated in the lower-right corner of each panel. The left panel shows unseparated splenocytes. The middle panel shows the twice-enriched fraction after three 6-minute magnetic separations plus an additional 6-minute separation of those enriched cells. The right panel shows the positive fraction.