BD IMagTM Mouse T Lymphocyte Enrichment Set-DM

Description

Enrichment of T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse T Lymphocyte Enrichment Set - DM Cat. No. 557793 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553061/553062 to detect T lymphocytes and a mixture of PE Anti-Mouse CD11b Cat. No. 557397/553311 , Anti-Mouse CD45/B220 Cat. No. 553089/553090 , Anti-Mouse 49b Cat. No. 558759 , and Anti-Mouse Ter-119/Erythroid Cells Cat. No. 553673 monoclonal antibodies to detect non-T leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart on the next page to identify the cell populations represented in this figure. The percentage of T cells is indicated in the lower-right corner of each panel. The far left panel shows unseparated splenocytes. The middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. The middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in the middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. The far right panel shows the positive fraction. Enrichment of T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse T Lymphocyte Enrichment Set - DM Cat. No. 557793 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553061/553062 to detect T lymphocytes and a mixture of PE Anti-Mouse CD11b Cat. No. 557397/553311 , Anti-Mouse CD45/B220 Cat. No. 553089/553090 , Anti-Mouse 49b Cat. No. 558759 , and Anti-Mouse Ter-119/Erythroid Cells Cat. No. 553673 monoclonal antibodies to detect non-T leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart on the next page to identify the cell populations represented in this figure. The percentage of T cells is indicated in the lower-right corner of each panel. The far left panel shows unseparated splenocytes. The middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. The middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in the middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. The far right panel shows the positive fraction. Show More Enrichment of T lymphocytes from mouse spleen. BALB/c splenocytes were labeled with the BD IMag Mouse T Lymphocyte Enrichment Set - DM Cat. No. 557793 and separated on the BD IMag Cell Separation Magnet Cat. No. 552311 according to the accompanying protocol. To demonstrate the efficiency of the enrichment, cells were stained with FITC Hamster Anti-Mouse CD3e Cat. No. 553061/553062 to detect T lymphocytes and a mixture of PE Anti-Mouse CD11b Cat. No. 557397/553311 , Anti-Mouse CD45/B220 Cat. No. 553089/553090 , Anti-Mouse 49b Cat. No. 558759 , and Anti-Mouse Ter-119/Erythroid Cells Cat. No. 553673 monoclonal antibodies to detect non-T leukocytes and erythrocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Please refer to the Enrichment Flow Chart on the next page to identify the cell populations represented in this figure. The percentage of T cells is indicated in the lower-right corner of each panel. The far left panel shows unseparated splenocytes. The middle left panel shows the combined enriched fraction after three 6-minute magnetic separations. The middle right panel shows the twice-enriched fraction after an additional 6-minute separation of the cells shown in the middle left panel. This additional incubation can result in up to 5 % increased purity with up to a 5 % decrease in recovery. The far right panel shows the positive fraction.