BD IMagTM Streptavidin Particles Plus - DM

Description

Figure 1. Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD IMag Mouse Hematopoietic Progenitor Enrichment Set - DM Cat. No. 558451 containing Biotin Anti-Mouse CD3, Anti-Mouse CD11b, Anti-Mouse CD45R/B220, Anti-Mouse Ly-6G and Ly-6C, and Anti-Mouse Erythroid Cells mAbs. After washing, the cells were labeled with BD IMag Streptavidin Particles Plus - DM Cat. No. 557812 at 50 mul/1x107 total cells. The labeled cells were then separated on a BD IMag Cell Separation Magnet Cat. No. 552311 . Unmanipulated bone marrow cells and the final depleted fraction were stained with APC Anti-Mouse CD117 Cat. No. 553356 to detect hematopoietic progenitors, and with PE Anti-Mouse TER-119 Cat. No. 553673 , CD45R/B220 Cat. No. 553089/553090 , and CD11b Cat. No. 557397/553311 mAbs to detect lineage-commited cells. The percentage of positive cells is indicated in each panel; placement of each marker is based upon staining with the appropriate isotype control data not shown . The final depleted fraction contains an increased proportion of CD117+ cells and less than 5 % of lineage-positive contaminants. Figure 2. Positive selection of human CD3-positive lymphocytes. Peripheral blood mononuclear cells PBMC were labeled with Biotin Anti-Human CD3 mAb Cat. No. 555338 and BD IMag Streptavidin Particles - DM at 20 mul/107 total cells. After labeling, the cells were separated using the Cell Separation Magnet, and the negative CD3 and positive CD3+ fractions were collected. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh PBMC left panel , the negative fraction middle panel , and the positive fraction right panel were stained with PE Anti-human CD3 mAb Cat. No. 555333 . The percent CD3+ cells in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Figure 1. Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD IMag Mouse Hematopoietic Progenitor Enrichment Set - DM Cat. No. 558451 containing Biotin Anti-Mouse CD3, Anti-Mouse CD11b, Anti-Mouse CD45R/B220, Anti-Mouse Ly-6G and Ly-6C, and Anti-Mouse Erythroid Cells mAbs. After washing, the cells were labeled with BD IMag Streptavidin Particles Plus - DM Cat. No. 557812 at 50 mul/1x107 total cells. The labeled cells were then separated on a BD IMag Cell Separation Magnet Cat. No. 552311 . Unmanipulated bone marrow cells and the final depleted fraction were stained with APC Anti-Mouse CD117 Cat. No. 553356 to detect hematopoietic progenitors, and with PE Anti-Mouse TER-119 Cat. No. 553673 , CD45R/B220 Cat. No. 553089/553090 , and CD11b Cat. No. 557397/553311 mAbs to detect lineage-commited cells. The percentage of positive cells is indicated in each panel; placement of each marker is based upon staining with the appropriate isotype control data not shown . The final depleted fraction contains an increased proportion of CD117+ cells and less than 5 % of lineage-positive contaminants. Figure 2. Positive selection of human CD3-positive lymphocytes. Peripheral blood mononuclear cells PBMC were labeled with Biotin Anti-Human CD3 mAb Cat. No. 555338 and BD IMag Streptavidin Particles - DM at 20 mul/107 total cells. After labeling, the cells were separated using the Cell Separation Magnet, and the negative CD3 and positive CD3+ fractions were collected. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh PBMC left panel , the negative fraction middle panel , and the positive fraction right panel were stained with PE Anti-human CD3 mAb Cat. No. 555333 . The percent CD3+ cells in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system. Show More Show More Figure 1. Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD IMag Mouse Hematopoietic Progenitor Enrichment Set - DM Cat. No. 558451 containing Biotin Anti-Mouse CD3, Anti-Mouse CD11b, Anti-Mouse CD45R/B220, Anti-Mouse Ly-6G and Ly-6C, and Anti-Mouse Erythroid Cells mAbs. After washing, the cells were labeled with BD IMag Streptavidin Particles Plus - DM Cat. No. 557812 at 50 mul/1x107 total cells. The labeled cells were then separated on a BD IMag Cell Separation Magnet Cat. No. 552311 . Unmanipulated bone marrow cells and the final depleted fraction were stained with APC Anti-Mouse CD117 Cat. No. 553356 to detect hematopoietic progenitors, and with PE Anti-Mouse TER-119 Cat. No. 553673 , CD45R/B220 Cat. No. 553089/553090 , and CD11b Cat. No. 557397/553311 mAbs to detect lineage-commited cells. The percentage of positive cells is indicated in each panel; placement of each marker is based upon staining with the appropriate isotype control data not shown . The final depleted fraction contains an increased proportion of CD117+ cells and less than 5 % of lineage-positive contaminants. Figure 2. Positive selection of human CD3-positive lymphocytes. Peripheral blood mononuclear cells PBMC were labeled with Biotin Anti-Human CD3 mAb Cat. No. 555338 and BD IMag Streptavidin Particles - DM at 20 mul/107 total cells. After labeling, the cells were separated using the Cell Separation Magnet, and the negative CD3 and positive CD3+ fractions were collected. Please refer to the Positive Selection Flow Chart to identify the separated cell populations represented in this figure. For flow cytometric analysis, fresh PBMC left panel , the negative fraction middle panel , and the positive fraction right panel were stained with PE Anti-human CD3 mAb Cat. No. 555333 . The percent CD3+ cells in each sample is given. Flow cytometry was performed on a BD FACSCalibur flow cytometry system.